Skip to main content
. 2019 Aug 5;8(8):836. doi: 10.3390/cells8080836

Figure 2.

Figure 2

Identification of the nuclear localization sequence (NLS) in PARN. (A) A schematic model of domain compositions of human PARN. The position of the NLS is labeled. (B) Verification of the putative PARN-NLS(523–540) by confocal microscopy analysis of Chinese-hamster ovary (CHO) cells transiently transfected with plasmids containing EGFP-fused PARN and the two truncated forms. The represented images show the results of EGFP fused to the N-terminus of PARN, and similar results were obtained for EGFP fused to the C-terminus of PARN (data not shown). The nuclei were counterstained with Hoechst 33342. (C) PARN-NLS(523–540) directed the nucleoplasm but not nucleoli localization of PARN. The nucleoli were marked by nucleophosmin (NPM1) using mouse anti-NPM1 antibody (red). Endogenous PARN was immunostained by rabbit anti-PARN antibody (green). In panels B and C, the scale bars represent 20 µm and the insets show the magnified region indicated by the arrows.