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. 2019 Aug 5;8(8):836. doi: 10.3390/cells8080836

Figure 6.

Figure 6

Phosphorylation-mimic S557D mutation induces structure switch of PARN-CTD. (A) far-UV CD spectra of PARN and PARN-S557D. (B) Trp fluorescence spectra of PARN and PARN-S557D. (C) Effect of S557D on the deadenylase activity of PARN. (D) Far-UV CD spectra of PARN-CTD(541–639) and PARN-CTD(541–639)-S557D. (E) Trp fluorescence spectra of PARN-CTD(541–639) and PARN-CTD(541–639)-S557D. (F) A schematic model of the structure switch induced by the S557D mutation. The S557mutation disrupts the local structure shielding the W639 fluorophore and facilitates the binding of CTD with the main body of PARN.