Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 7;8(8):850. doi: 10.3390/cells8080850

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Analysis of protein aggregation in ubi1∆ub-HA and SMT3-S-eL40A-HA cells. Strains W303-1A (Wild type), JDY532 (ssb1∆ ssb2∆), JDY923 (ubi2∆), SMY113 (UBI1-HA), SMY256 (UBI1-HA ubi2∆), SMY257 (ubi1∆ub-HA ubi2∆), and SMY258 (SMT3-S-eL40A-HA ubi2∆) were grown to logarithmic phase in YPD medium at 30 °C. Then, total protein extracts and protein aggregates were prepared, separated by SDS-PAGE, and visualized by Coomassie staining (upper part) or subjected to western blot analysis (lower part) using antibodies detecting either the HA-tag of the eL40-HA protein or the uL29, uS3, and Pgk1 proteins.