Figure 5.

Wistar rats were transplanted with 5 × 10⁷ cells/kg HHALPCs, by intraportal injection, with or without (PBS) anticoagulant treatment, i.e., the combination of heparin and bivalirudin (hep-biva) or a high dose of heparin (hep 300 IU). (A–C) Blood samples were taken before and at 1 h after transplantation. Total blood count, coagulation factors and TAT levels were analyzed and were expressed relative to basal levels before cell infusion (in %). Bars represent medians with interquartile ranges (n = 6/group). Mann–Whitney test (* p < 0.05, ** p < 0.01, *** p < 0.001). (D) Liver sections were analyzed at 1 h after cell infusion by phospho-tungstic acid-hematoxylin stain (PTAH) and human beta1 integrin staining (B1 Int.). Objective 10×. (E) Quantification of the number of portal veins (PVs) that contained cells and fibrin, expressed as percentage of the total PVs containing cells. Bars represent medians with interquartile ranges (n = 6). Mann–Whitney test (** p < 0.01).