Figure 1.

Establishment of a post-chronic Cd-exposed human lung cell line and evaluation of its Cd-resistance and DNA damage and repair capacity. (A) Schematic of the establishment of chronic and post-chronic Cd-exposed BEAS-2B cell line used in the previous [19] and current study; the post-chronic Cd-exposed BEAS-2B cells are termed as “CR0” while the passage-matched control cells are termed as “PM”. (B,C) Cell proliferation rate of CR0 and PM cells grown in standard LHC-9 medium or culture medium containing 20 µM CdCl₂ was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Results are representative of at least three independent experiments, and error bars represent mean ± SD of at least three technical replicates. (D) Colony formation assay of PM and CR0 cells treated with 20 µM of CdCl₂ and grown for 7–10 days.