Figure 2.

Evaluation of DNA damage and repair capacity in the post-chronic Cd-exposed cell line. (A) Intrinsic DNA damage level of CR0 cells (over PM control cells) was measured by comet assay (n = 6); two DNA damage parameters, % tail DNA and tail extent moment, are shown. (B) Representative images of comet assay in PM and CR0 cells stained with 5 μg/mL of propidium iodide after gel electrophoresis. (C) Expression of histone H2AFX and phosphorylated H2AFX (γH2AFX) were determined by Western blot (see Figure S2 for full blot). (D,E) Cell viability of PM and CR0 cells exposed to various concentrations of DNA damage-inducing agent H₂O₂ for 2 h without or with a 24 h recovery period was measured by MTS assay; results were representative of at least three independent experiments, and error bars represent mean ± SD of at least three technical replicates. (F) Tail extent moment of PM and CR0 cells exposed to 0, 2.5, and 10 µM of H₂O₂ for 2 h without or with a 24 h recovery period was analyzed by comet assay. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.