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. 2019 Aug 17;8(8):919. doi: 10.3390/cells8080919

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the CXCL10 promoter regulation in response to IFNβ + TNF vs. IFNβ. (A) Schematic representation of the CXCL10 promoter (CXCL10prom) luciferase constructs used in this study indicating the main transcription factors consensus binding sites. (B) U3A and U3A-STAT1 cells were transfected with the indicated CXCL10prom-luciferase reporter constructs and either left untreated or stimulated with IFNβ or IFNβ + TNF. Relative luciferase activities were measured at 16 h post-stimulation and expressed as fold over the corresponding unstimulated condition. Mean +/− SEM, n = 6. Statistical analyses were performed using an unpaired t-test comparing each promoter to the CXCL10prom-972bp construct. p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****).