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. 2019 Sep 3;8:e46007. doi: 10.7554/eLife.46007

Figure 6. Suppression of Nfatc1 promotes enlargement of the thoracic duct.

(a,b) Confocal images of the mid-trunk of 5 dpf Tg(mrc1a:eGFP)y251, Tg(kdrl:mCherry)y171 double-transgenic control MO (a) or nfatc1 splice MO (b) injected animals. The dashed boxes in panels a and b show the areas magnified in panels c and d, respectively. (c,d) Magnified images from panels a and b, with the thoracic duct pseudocolored in green and other vessels in gray. (e) Quantitation of thoracic duct size measured as the area encompassed by the thoracic duct in confocal images of the same seven mid-trunk somitic segments in five dpf wildtype (n = 9) and nfatc1 MO-injected (n = 9) animals. (f) Sequence alignment of wildtype and nfatc1△8/△8 mutant genomic DNA. Schematic of nfatc1 protein domains, CRISPR target site, and truncated mutant nfatc1 polypeptides. (g,h) Confocal images of the mid-trunk of 5 dpf Tg(mrc1a:eGFP)y251 wildtype (g) or nfatc1△8/△8 mutant (h) animals. (i,j) Magnified images from panels g and h, with the thoracic duct pseudocolored in green and other vessels in gray. (k) Quantitation of thoracic duct size measured as the area encompassed by the thoracic duct in confocal images of the same seven mid-trunk somitic segments in five dpf wildtype (n = 8) and nfatc1△8/△8 mutant (n = 10) animals. Rostral is to the left in all images. Scale bar = 100 μm (b,d,g,i). All graphs are analyzed by t-test and the mean ± SD is shown.

Figure 6—source data 1. Numerical data for Figure 6 and Figure 6—figure supplement 1.
elife-46007-fig6-data1.xlsx (10.1KB, xlsx)
DOI: 10.7554/eLife.46007.023

Figure 6.

Figure 6—figure supplement 1. Suppressing nfatc1 promotes thoracic duct enlargement.

Figure 6—figure supplement 1.

(a,b) Confocal images of the mid-trunk of 5 dpf Tg(mrc1a:eGFP)y251, Tg(kdrl:mCherry)y171 control (a) and nfatc1 ATG MO-injected (b) animals. The dashed boxes in panels a and b show the areas magnified in panels c and d, respectively. (c,d) Magnified images from panels a and b, with the thoracic duct pseudocolored in green and other vessels in gray. (e) Quantitation of thoracic duct size measured as the area encompassed by the thoracic duct in confocal images of the same seven mid-trunk somitic segments in five dpf wildtype (n = 11) and nfatc1 MO-injected (n = 9) animals. (f,g) Confocal images of the mid-trunk of 5 dpf DMSO (f) and cyclosporine A (g) treated animals. The dashed boxes in panels f and g show the areas magnified in panels h and i, respectively. (h,i) Magnified images from panels f and g, with the thoracic duct pseudocolored in green and other vessels in gray. (j) Quantitation of thoracic duct size measured as the area encompassed by the thoracic duct in confocal images of 7 somitic segments from five dpf DMSO (n = 16) and cyclosporine A (n = 17) treated animals. All images are lateral views, rostral to the left. Scale bar: 100 μm (a,c). All graphs are analyzed by t-test and the mean ± SD is shown.