Appendix 1—figure 2. Seeded aggregation experiments at low monomer concentrations designed to estimate the concentration of monomeric $\alpha$-synuclein in equilibrium with fibrils.

The seed concentration is in all cases 0.75 μM and the ThT concentration is 10 μM. The experiment was performed at room temperature in order to slow the reaction down and avoid temperature effects on the fluorescence upon introduction of the multiwell plate into the fluorescence platereader.