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. 2019 Aug 14;11(8):1168. doi: 10.3390/cancers11081168

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Production and initial biochemical characterization of the conjugates. (A) Schematic representation of the proteins. (B) Conjugates after final RP-HPLC purification were analyzed on a 4%–12% SDS-PAGE gel under reducing conditions. The numbers to the left are the molecular weight (kDa) of the marker proteins in lane M. (C) Analytical size-exclusion chromatography profiles of the conjugates. The numbers above the chromatograms are the molecular weight (kDa) of protein standards. (D) RP-HPLC analysis of the conjugates during a 20 min linear gradient from 30% to 60% acetonitrile in water with 0.1% TFA.