Table 1.
Conjugates | Purity (%) a | Calc. Mw (Da) | Found Mw (Da) b |
---|---|---|---|
(ZHER2:2891)2–ABD–MC-DM1 | >95 | 21,006.3 | 21,005.8 |
(ZHER2:2891)2–ABD–E3–MC-DM1 | >95 | 21,393.6 | 21,393.0 |
(ZHER2:2891)2–ABD–E6–MC-DM1 | >95 | 21,781.0 | 21,780.1 |
(ZHER2:2891)2–ABD–IAA | >95 | 20,219.9 | 20,219.5 |
a Determined by analytical RP-HPLC; b Mass spectrometry was used to determine the molecular weight (Mw) of the conjugates. Deconvolution was performed to determine the monoisotopic molecular weight of the proteins.