Figure 4.

Effect of PAR2 knockdown on TGF-β1-induced growth inhibition in Panc1 cells. (A) Panc1 cells were transfected twice with 50 nM of either PAR2 siRNA or Co siRNA. Forty-eight hours after the second round of transfection cells were treated, or not, with TGF-β1 for 24 h, then detached and counted. Data are the mean ± SD of three experiments and are displayed as % reduction in cell numbers of TGF-β1-treated cells relative to numbers of untreated control cells. The asterisk indicates a significant difference. (B) As in (A) except that cells were lysed after TGF-β1 treatment and processed for immunoblotting of ALK5, and β-actin as a loading control.