Figure 1.

Characteristics of the microfluidic enrichment procedure and of the tumor cell lines used for the spiking experiments are illustrated. (a) Four SCLC tumor cell lines (BHGc26, BHGc10, BHGc16, and BHGc7) were fluorescently labeled and spiked into blood (100 cells per 10 mL) in triplicates. The graph depicts the mean percentage and the standard deviation of tumor cells captured in the Parsortix™ microfluidic cassette. (b) The gene expression levels of the epithelial and neuroendocrine cell lineage specific markers of the same cell lines are shown relative to the expression level of cyclin dependent kinase inhibitor 1B as reference gene. The graphs depict the means and the standard deviation from duplicate qPCRs amplifications.