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. Author manuscript; available in PMC: 2019 Sep 3.
Published in final edited form as: J Biol Chem. 1997 Jul 18;272(29):18179–18184. doi: 10.1074/jbc.272.29.18179

FIG. 3. Internalization of 125I-BH-CCK-8 by NIH/3T3 cells stably expressing WT or mutant CCK receptors.

FIG. 3.

NIH/3T3 cells expressing the indicated WT or mutant CCKAR (left panel) or CCKBR (right panel) were incubated with 125I-BH-CCK-8 (50 pM). At the indicated time points, cells were subjected to a 0.5 M KSCN solution. Internalized radioligand is defined as the 125I-BH-CCK-8 that could not be stripped by KSCN and is expressed as percent of the total saturably bound 125I-BH-CCK-8 in control cells processed in parallel without KSCN exposure. Each value represents the mean ± S.E. of at least three experiments performed in duplicate.