Figure 4.

An OGT target, eIF4G1, is reduced in OGT-deficient β-cells. Glycosylation of MIN6 eIF4G1 by sWGA precipitation + eIF4G1 immunoblot (A) and RL2 IP (versus mouse IgG control) + eIF4G1 immunoblot (B) or eIF4G1 IP (versus rabbit IgG) + eIF4G1 and RL2 immunoblot (C). The blots are representative of two independent experiments. eIF4G1 levels in 4–5–week–old OGT f/y (n = 5) and βOGTKO (n = 4) mice, shown for immunoblot protein (relative to vinculin, normalized to OGT f/y avg.) (D and E) and qPCR gene expression (relative to β-actin, log₂-fold change to OGT f/y avg.) (n = 3) (F). Statistical analyses were conducted using unpaired, two-tailed Student's t test; *, p < 0.05.