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. 2019 Jul 19;294(35):12892–12900. doi: 10.1074/jbc.RA119.009602

Figure 2.

Figure 2.

The role of dMPPE in regulating light-induced Rh1 endocytosis is independent of its phosphoesterase activity. A, Western blots show the expression of dMPPE164–183A and the molecular weight of Rh1 in cn,dmppe,bw;rh1-GAL4/p[UAS-dmppe164–183A] flies. B, ERVs in cn,dmppe,bw mutant and cn,dmppe,bw;rh1-GAL4/p[UAS-dmppe164–183A] flies. Cross-sections of the eye were stained with a monoclonal Rh1 antibody. Scale bar, 2 μm. C, quantification of the number of ERVs per ommatidium for each genotype and treatment. Data are presented as the mean ± S.D. WT, dark: 34 sections from 14 flies; light 30 min: 31 sections from 14 flies; dmppe, dark: 33 sections from 12 flies; light 30 min: 32 sections from 12 flies; dmppe;rh1>dmppe164–183A, dark: 6 sections from 3 flies; light 30 min: 12 sections from 6 flies. The scatter plots for WT flies and dmppe mutants are also presented in Figs. 1B and 4C. ***, p < 0.001.