Figure 4.

Arr1/dMPPE interaction regulates Rh1 endocytosis. A, Western blots show the molecular weight of Rh1 in cn,dmppe,bw;rh1-GAL4/p[UAS-dmppe^344–345A] flies. B, ERVs in dmppe mutants expressing mutant dMPPE^344–355A were induced by exposure to light for 30 min. Cross-sections of the eye were stained with a monoclonal Rh1 antibody. Scale bar, 2 μm. C, quantification of the number of ERVs per ommatidium for each genotype and treatment. Data are presented as the mean ± S.D. WT, dark: 34 sections from 14 flies; light 30 min: 31 sections from 14 flies; dmppe, dark: 33 sections from 12 flies; light 30 min: 32 sections from 12 flies; dmppe;rh1>dmppe^344–355A, dark: 6 sections from 3 flies; light 30 min: 8 sections from 4 flies. The scatter plots for WT flies and dmppe mutants are also presented in Figs. 1B and 2C. n.s., no significance; ***, p < 0.001.