Figure 2.

Binding interactions of FITC-labeled sGCβ1(1–358) mutant proteins with HSP90. Each indicated FITC-labeled sGCβ protein (0.5 μm) was titrated with increasing concentrations of HSP90, and the residual polarization change was recorded and plotted. The points depict mean ± S.D. (error bars) for three samples and are representative of two independent experiments. Fit lines are red, and the resulting binding constant estimates are indicated.