Figure 6.

Association of each sGCβ protein with sGCα or HSP90 in heme-deficient mammalian cells. Heme-deficient COS-7 cells were transfected to express each sGCβ1(1–619) mutant and sGCα for 48 h. Supernatant aliquots (equal protein) were immunoprecipitated with an anti-sGCβ antibody followed by SDS-PAGE and Western blot analysis (IB) with anti-HSP90 and sGCα antibodies. A, representative Western blot analysis of bound sGCα, HSP90, and sGCα retained on the beads. B, densitometric quantifications of HSP90 or sGCα associated with each sGCα protein. Results are the mean ± S.D. (error bars) of three independent experiments.