(A and B) Ethanol sedation time courses of control flies (w1118 from BDSC) and the indicated Mef2 genotypes. Compound heterozygous Mef2 mutants Mef230−5/Mef265 (A) and Mef225−34/Mef265 (B) took longer to become sedated than flies heterozygous for these alleles. (C, D) ST50 values, derived from the data in panels A and B, respectively. There was a significant overall effect of Mef2 genotype on ST50 values (individual one-way ANOVAs; panel C, p<0.0001; panel D, p<0.0001; n=10). ST50 values were greater in Mef230−5/Mef265 (C) and Mef225−34/Mef265 (D) flies than in all other genotypes (*BMCs; panel C, p≤0.0003; panel D, p<0.0001).