Table 1.
Primary antibodies
| Molecular marker | Immunogen | Host | Dilution | Clone | Source | RRID |
|---|---|---|---|---|---|---|
| Albumin | whole mouse albumin | goat | 1:800 | poly | Bethyl, A90–234A | RRID:AB_67122 |
| claudin5 clone 4C3C2, Alexa488 | Synthetic peptide from the mouse claudin5 | mouse | 1:10000 | mono | Invitrogen, 352588 | RRID:AB_2532189 |
| Connexin 43 (Cx43s) | PSSRASSRASSRPRPDDLEI | rabbit | 1:2000 | poly | Sigma, C6219 | RRID:AB_476857 |
| Connexin 43 (Cx43a) | (C)HAQPFDFPDDNQNSK | rabbit | 1:10000 | poly | Alomone, ACC-201 | RRID:AB_10917597 |
| Connexin 40 | (C)GHRFPQGYHSDKR | rabbit | 1:3000 | poly | Alomone, ACC-205 | RRID:AB_2340916 |
| Connexin 37 | (C)EHQMAKISVAEDGR | rabbit | 1:2000 | poly | Alomone, ACC-204 | RRID:AB_2722598 |
| Smooth muscle actin, SMA, 1A4 | N-terminal synthetic decapeptide of α-SMA | mouse | 1:1000 | mono | Sigma, A5228 | RRID:AB_262054 |
| Glial fibrillary acidic protein, GFAP | purified bovine GFAP | chicken | 1:3000 | poly | Millipore, AB5541 | RRID:AB_177521 |
| CD31/PECAM-1 | Mouse myeloma cell NS0-derived recombinant mouse CD31 Glu18-Lys590 | goat | 1:6000 | poly | RD, AF3628 | RRID:AB_2161028 |