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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Nat Genet. 2019 Aug 19;51(9):1380–1388. doi: 10.1038/s41588-019-0479-7

Figure 4. Silencing of HERV-H sequences weakened the TAD boundaries in hESC.

Figure 4.

(a) Design of the CRISPR-dCas9-KRAB system to silence HERV-H expression using sgRNAs targeting 5’ LTR7. (b) Gene expression values of HERV-H1 and HERV-H2 in WT and CRISPRi-targeted hESCs. (c) Hi-C interaction matrices of the CRISPRi targeted hESC lines (sgHERV-H1 and sgHERV-H2) are shown, along with DI scores, and log2(fold-change) of gene expression in engineered cell lines over control at the HERV-H1 and HERV-H2 loci.

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