Figure 1:

A: Schematic representation of chronic intermittent ethanol (CIE) exposure paradigm. Mice were exposed to ethanol for 16 hr each day for 4 consecutive days followed by a 3-day withdrawal period. This 7-day protocol was repeated for 4 weeks. During the latter 2 weeks, mice were injected twice daily with either 1g/kg lactate or saline vehicle upon entering and removal from the vapor chambers. Blood was drawn 8 hr following the final ethanol exposure, and brains were removed for metabolic analysis 72 hr after the final vapor exposure. B: Time course of blood ethanol concentration during and after the final vapor exposure of the CIE exposure paradigm shown in (A). Blood ethanol concentrations were maintained at approximately 200 mg/dl for the duration of ethanol exposure and returned to 0 mg/dl prior to 8 hr after removal from the vapor chamber. n=5.