Table 1.
Showing the primer sequences using for gene methylation and mRNA expression studies.
| Primer Name | Sequence |
|---|---|
| hPER1 MFP | 5’ ATTTAGGTTTACGTGCGTTC 3’ |
| hPER1 MRP | 5’ CGACTCAAAAACGAAAATCG 3’ |
| hPER1 UMFP | 5’ TAGTATTAGTATTTAGGTTTATGTGTGTTT 3’ |
| hPER1 UMRP | 5’ AACAACAATCCAACTCAAAAACAAAAATCA 3’ |
| hPER2 MFP | 5’ GCGGTTTCGTTGCGGTTTAC 3’ |
| hPER2 MRP | 5’ GCCGACGCCGTTTCAAACCG 3’ |
| hPER2 UMFP | 5’ GTGGTGTGGTGTGGTTTTGTTGTGGTTTAT 3’ |
| hPER2 UMRP | 5’ ACACCCCCACACCAACACCATTTCAAACCA 3’ |
| hPOMC MFP | 5’ ACGGGGGTGTTAAGTTTTTC 3’ |
| hPOMC MRP | 5’ ACTATCCTACCGAAAACGCA 3’ |
| hPOMC UMFP | 5’ TATATGGGGGTGTTAAGTTTTTT 3’ |
| hPOMC UMRP | 5’ CACTATCCTACCAAAAACACACC 3’ |
| hPER2 BSP FP | 5’ GTGTGTTTTTGGTTTTGTTTTAGGT 3’ |
| hPER2 BSP RP | 5’-/5Bio/TTAATTTCCTTAACCTTCCCCATAC 3’ |
| hPER2 seq FP | 5’ GAGTGGTCGAGTCGCGCGTAG 3’ |
| hPOMC BSP FP | 5’GTTGGGGTTAAAAATAGGTGGT 3’ |
| hPOMC BSP RP | 5’-/5Bio/TCTATAAAAACCCTCCCTACCC 3’ |
| hPOMC seq FP | 5’GGAGTTGTTTTAGATTTT 3’ |
| hPER2 RT FP | 5’ TCCAGTGGACATGAGACCAA 3’ |
| hPER2 RT RP | 5’ CGCTACTGCAGCCACTTGTA 3’ |
| hPOMC RT FP | 5’ AAGCGCTACGGCGGTTTC 3’ |
| hPOMC RT RP | 5’ TGATGATGGCGTTTTTGAACA 3’ |
| hGAPDH RT FP | 5’ AACCTGCCAAATATGATGAC 3’ |
| hGAPDH RT RP | 5’ ATACCAGGAAATGAGCTTGA 3’ |
| h18S RT FP | 5’ AAACGGCTACCACATCCAAG 3’ |
| h18S RT RP | 5’ CCTCCAATGGATCCTCGTTA 3’ |
| hRPL19 RT FP | 5’ ATCGATCGCCACATGTATCA 3’ |
| hRPL19 RT RP | 5’ GCGTGCTTCCTTGGTCTTAG 3’ |
Forward primer (FP), Reverse primer (RP), Methyl forward primer (MFP), Methyl reverse primer (MRP), Un methyl forward primer (UMFP), Un methyl reverse primer (UMRP).