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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Eur J Immunol. 2019 Jun 19;49(9):1399–1414. doi: 10.1002/eji.201847988

Figure 2. Screening of cross-reactive CD8+ T cell epitopes of Mtb by Mtb85B peptides.

Figure 2.

Induction of Mtb85B-specific polyfunctional CD8+ and CD4+ T cells by immunization consisting of priming with the rBCG-Mkan85B and boosting with DNA-Mkan85B three times in BALB/c (A) or C57BL/6 mice (B). Spleen cells were incubated with pooled (A, B) and individual (C, D) 15-mer overlapping peptides of Mtb85B for induction of polyfunctional CD8+ and CD4+ T cells as described in Materials and Methods. (E) Epitope mapping of Mtb85B peptide for induction of polyfunctional CD8+ T cells stimulated with 9-mer peptide in BALB/c mice immunized with rBCG-Mkan85B followed by DNA-Mkan85B boost. Polyfunctional epitope-sequences were termed as pep8 peptide with YYQSGLSIV and pep9 peptide with YQSGLSIVM sequences. H113 is a screened 15-mer peptide which contains both the pep8 and pep9 epitope sequences. Data represent three to five independent experiments with five mice per group. Error bars represent SEM.