Figure 4. Augmented induction of Mtb85B-specific polyfunctional CD8⁺ T cells by co-inducing CD4⁺ T cells in CB6F1 mice.

(A) Representative fluorogram of epitope-specific polyfunctional CD8⁺ and CD4⁺ T cell-inductions in splenocytes from C57BL/6, BALB/c and CB6F1 mice, with TNF-Alexa Fluor 488 on x-axis and IFN-γ-PE on y-axis. (B) Augmented induction of the polyfunctional CD8⁺ T cells in H2^b/d CB6F1 mice. Polyfunctional CD8⁺ T cell induction by stimulation with 9-mer-peptides in BALB/c mice immunized with the regimen (left panel). C57BL/6 mice immunized with the prime boost regimen induce polyfunctional CD4⁺ T cells by stimulation with 15-mer-peptides (middle panel). The polyfunctional CD8⁺ T cell inductions were augmented by co-inducing the polyfunctional CD4⁺ T cells in the CB6F1 mice by rBCG-Mkan85B priming followed by boosting with DNA-Mkan85B three times (right panel). Data represent three independent experiments with five mice per group. Error bars represent SEM.