(A) Torsional stiffness did not differ between sham, 1x, and 3x six weeks after injury, but torsional stiffness was significantly greater in male IVDs than female IVDs after 3x injury (p < 0.05). (B) Torque range exhibited a similar pattern to torsional stiffness, but the sex difference after 3x injury was not significant (p = 0.058). Neither torsional hysteresis (C), torsional neutral zone length (D), nor torsional neutral zone stiffness (E) differed between groups six weeks after annular puncture. (n = 5–8, p values for 2-way ANOVA effects shown below each graph).