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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 Mar 4;30(12):2460–2469. doi: 10.1007/s13361-019-02139-0

Figure 4.

Figure 4.

Offline MS/MS analysis localizing the PTM sites, tri-methylation at Ala1, phosphorylation at Ser15 and Ser16 for rhesus macaque MLC-2F. Representative ECD and CAD fragment ions and sequence maps of (a) mono-phosphorylated MLC-2F and (b) bis-phosphorylated MLC-2F. P, phosphorylation; (Me)3, tri-methylation; N.D., not detected; Red circles, theoretical isotopic abundance distribution of the isotopomer peaks of c and z˙ ions; Blue circle, loss of 1 Da from the c ions presumably resulting from H rearrangement [41].