Fig. 2.

Experimental design and biological endpoints. Human organotypic buccal and small airway cultures were exposed in an in vitro aerosol exposure system (the Vitrocell^® 24/48 exposure systems is illustrated). Three experimental phases, each including three independent aerosol/smoke generations, were performed using new batches of buccal and small airway cultures, totaling nine repetitions per culture type. Various endpoints were measured at specific time points before and after exposure, from each smoke/aerosol generation. PBS, phosphate-buffered saline