. 2019 Mar 5;14(6):863–883. doi: 10.1007/s11739-019-02055-x

Table 3.

Smoke and aerosol generation parameters

Item	Puff volume (mL)	Puff duration (s)	Frequency (puff per 60 s, per item)^a	Puff exhaust time (s)	Puff count per item^a	Puff profile
Test mix, base, carrier	55 (at the port of the e-cigarette aerosol collection machine)	5	2	8	Max of 140 puffs per MarkTen^® cartridge	Square shaped
3R4F reference cigarette^b	55	2	2	8	10–12 puffs per cigarette	Bell shaped

^aOne item refers to one MarkTen^® EC cartridge for test mix, base, or carrier EL; or one stick of 3R4F reference cigarette

^bHealth Canada (HC) smoking regimen with ventilation holes blocked [30]