Table 4.
Smoke and aerosol exposure doses
| Smoke or aerosol concentration (%) fed in to the exposure chambera | Duration of exposure (min) | Total puff numbers | Median concentration of deposited nicotine in the exposure chamber (µg nicotine/mL PBS) | |
|---|---|---|---|---|
| Buccal culture experiments | ||||
| Air (control for 3R4F) | 0b | 28 | 112 | NA |
| 3R4F concentration 1 | 13 | 28 | 112 | 18c |
| 3R4F concentration 2 | 24 | 28 | 112 | 33 |
| 3R4F concentration 3 | 69 | 28 | 112 | 131 |
| Air (control for EC aerosols) | 0b | 28 | 112 | NA |
| Test mix (undiluted) | 100 | 28 | 112 | 210c |
| Base (undiluted) | 100 | 28 | 112 | 216c |
| Carrier (undiluted) | 100 | 28 | 112 | NA |
| Small airway culture experiments | ||||
| Air (control for 3R4F) | 0b | 28 | 112 | NA |
| 3R4F concentration 1 | 3 | 28 | 112 | 2 |
| 3R4F concentration 2 | 7 | 28 | 112 | 9 |
| 3R4F concentration 3 | 13 | 28 | 112 | 18c |
| Air (control for EC aerosols) | 0b | 28 | 112 | NA |
| Test mix (undiluted) | 100 | 28 | 112 | 210c |
| Base (undiluted) | 100 | 28 | 112 | 216c |
| Carrier (undiluted) | 100 | 28 | 112 | NA |
PBS phosphate-buffered saline, NA not applicable
aValues refer to the smoke or aerosol concentration fed to the Vitrocell® 24/48 Dilution/Distribution Module
bControls refer to 100% air administered to the culture on the same exposure plate
cBecause identical doses were administered to both buccal and small airway cultures, the aggregated values (median concentrations) of deposited nicotine are indicated