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. 2019 Sep 3;9:12702. doi: 10.1038/s41598-019-49230-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of transfection with CEACAM1-4L/4S on invasion, lumen formation and tumor growth. Control; CEACAM1 vector control transfected NUGC3 (undifferebtiated type) and MKN7 (differentiated type) cells. Long; CEACAM1-4L transfected NUGC3 cells.Short; CEACAM1-4S transfected NUGC3 cells, shRNA; shRNA targeted to CEACAM1 transfected MKN7 cells. (A) RT-PCR analysis of NUGC3 cells with transfected vector control, CEACAM1-4L, CEACAN1-4S, and of MKN7 cells with transfected shRNA targeted to CEACAM. (B) In vitro invasion assay using Matrigel invasion chanbers. (C) Representative images of gastric cancer lumen formation in Matrigel 3D culture. a: no lumen formation, NUGC3 cells; b: lumen formation, NUGC3 cells; c: no lumen formation, MKN7 cells; d: lumen formation, MKN7cells. (D) Lumen formation in NUGC3 and MKN7 cells in 3D culture. n.s.; not significance. (E) In vivo, the subcutaneous tumor model with NOD/SCID mice. n.s.; not significance.