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. 2019 Sep 3;9:12674. doi: 10.1038/s41598-019-49081-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phyla identified by amplicon sequencing of the V3-V4 region of the 16S rRNA gene and percentage abundance based on normalized OTU numbers in the ileum, cecum and colon of 24 horses. Phyla with abundance above 0.1% in at least one compartment are shown separately. The group “Other” includes the phyla Elusimicrobia, Fusobacteria, Planctomycetes, SR1, TM7, and WPS-2 all with abundances below 0.1%. Samples from 21 horses were used to analysis of the microbial composition in the Ileum and from 24 horses for the Cecum and Colon. Significant differences were found between ileum and the hind gut (cecum/colon), but not between the cecum and colon in an ANOVA analysis. Phyla that differed significantly are marked with *(p < 0.05).