Table 4.
Efficiencies of the c-kit gene KI by CRISPR/Cas9.
| Injected materials | ssODN | No. of analysed | No. of intact blastomeres(%) | No. of modified blastomeres (%) | ||||
|---|---|---|---|---|---|---|---|---|
| Indel | KI | |||||||
| length | strand | embryos | blastomeres | Imprecise | precise | |||
| mRNA | 36 nt | sense | 5 | 49 | 17 (34.7) | 32 (65.3) | 0 (0) | 0 (0) |
| anti-sense | 5 | 37 | 11 (29.7) | 25 (67.6) | 1 (2.7) | 0 (0) | ||
| 100 nt | sense | 5 | 36 | 15 (41.7) | 21 (58.3) | 0 (0) | 0 (0) | |
| anti-sense | 5 | 39 | 19 (48.7) | 20 (51.3) | 0 (0) | 0 (0) | ||
| nuclease | 36 nt | sense | 5 | 38 | 0 (0) | 24 (63.2) | 2 (5.3) | 12 (31.6) |
| anti-sense | 5 | 42 | 5 (11.9) | 17 (40.5) | 17 (40.5) | 3 (7.1) | ||
| 100 nt | sense | 5 | 39 | 37 (94.9) | 2 (5.1) | 0 (0) | 0 (0) | |
| anti-sense | 5 | 34 | 29 (85.3) | 5 (14.7) | 0 (0) | 0 (0) | ||