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. 2019 Sep 3;9:12718. doi: 10.1038/s41598-019-49155-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Over-expression of TUSC2 mimics the effects of miR-138 knockdown: (A) Immunocytochemistry images of cells following antagomiR-138 transduction scale bar-20 µm. TUSC2 protein is showed in red and nuclei are stained in blue. B) Western blot analysis of TUSC2 protein in MDA-MB-231 cell line transduced with indicated lentivirus. β-Actin serves as loading control. (C) Representative bioluminescent images of NSG mice implanted with antagomiR-138 expressing or TUSC2 overexpressing or control MDA-MB-231 cells, at indicated time points post engraftment in mammary fat pads. (D) Xenograft tumour sizes in mice on day 24 are represented as bar graph from indicated group (n = 10). (E) Histogram representing relative transcript abundance of TUSC2 upon miR-138 knockdown in MDA-MB-231 cells, which express control shRNA or shRNA against TUSC2. (F) Knockdown of TUSC2 rescues the proliferation defect induced by miR-138 knockdown. Control refers to miR-138 knockdown. (G) TUSC2 knockdown decreases caspase 3/7 activity and rescues miR-138 phenotype. Control refers to miR-138 knockdown. Error bars represent the standard deviation (Student’s t-test; *[P < 0.05], **[P < 0.01], ***[P < 0.001]).