Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 3;9:12680. doi: 10.1038/s41598-019-49061-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

In vivo validation of p53 DBD Nbs. (a) Pull down of endogenous wild type p53 in HEK293T cells that transiently express FLAG-tagged p53 DBD Nbs. Crude lysates (1 mg) of transfected HEK293T cells were incubated with anti-FLAG M2 affinity gel, resulting in the immobilisation of the FLAG-tagged Nbs. As a negative control, HEK293T cells were transiently transfected with a FLAG-tagged GFP Nb (C). Co-precipitation of endogenous wild type p53 was observed for all p53 DBD Nbs, whilst it was absent for the negative control. (b) Expression levels of the transfected FLAG-tagged Nbs in crude lysates of HEK293T cells (40 µg). Nbs were expressed at similar levels. For reasons of clarity and conciseness, blots were cropped to the bands of interest. Full-length blots are depicted in Supplementary Fig. S10. (LC = light chain of IgG antibody).