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. 2019 Sep 3;9:12706. doi: 10.1038/s41598-019-48971-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CSC inhibits the phosphorylated CFTR activity in CR-HBE and following ligand-induced activation of CFBE. (a) Effect of CSC (200 μg/ml) or DMSO on forskolin activated I_sc in CR-HBE^WT/WT cells. (b) Summary CSC (200 μg/ml) or DMSO effect after 20 min on forskolin-stimulated CFTR-mediated I_sc. Each dot represents the average of 2–4 technical replicates per cell-line from five individuals. *p < 0.05 (c) Representative I_sc traces on CFBE after isoproterenol (Iso, delta concentration: 0.03, 0.1, 0.3, 1, and 10 μM), forskolin (0.01, 0.04, 0.1, 0.2, 1, 2, and 10 μM) or VIP (0.1, 0.3, 1.0, 3.0, 10, and 100 nM) stimulation. (d) Summary of maximal CFTR-mediated current stimulation by Isoproterenol (50 μM) and VIP (100 nM) compared to maximal forskolin stimulation (10 μM). Data are means ± SEM, n = 3 of technical duplicates *p < 0.05 (e) Representative I_sc traces and summary figures (right panels, n = 3 with duplicates) of the β-adrenoreceptor agonist, isoproterenol activation (upper panels) or (f) VIP stimulation followed by CSC (200 μg/ml, black line) or DMSO administration.