Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 3;9:12692. doi: 10.1038/s41598-019-49070-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Comparison of intracellular calcium assays for the endogenous muscarinic receptor expressed in 293-F cells. Time-courses during stimulation with 30 nM ACh for cells labelled with (a) stable expression of GCaMP6s and (b) the synthetic indicator fluo-4. Black lines indicate n = 12 negative control wells (0.5% DMSO only); blue lines indicate n = 4 control wells treated with 10 µM ionomycin; red lines indicate n = 12 positive control wells pre-incubated with an inhibitor (1 µM scopolamine). (c,d) Normalized response as a function of concentration of selected antagonists. (e,f) Normalized response as a function of concentration of selected agonists. Data are expressed as mean and standard deviation of all individual data points from three separate experiments. Lines are Hill function fits to the data.