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. 2019 Sep 3;9:12692. doi: 10.1038/s41598-019-49070-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of intracellular calcium assays for TRPV1 receptors transiently expressed in 293-F cells. Time-courses for cells during stimulation with 150 nM capsaicin labelled with (a) stable expression of GCaMP6s and (b) the synthetic indicator fluo-4. Black lines indicate n = 12 negative control wells (0.5% DMSO only); blue lines indicate n = 4 control wells treated with 2 mM calcium; red lines indicate n = 12 positive control wells pre-incubated with an inhibitor (10 µM capsazepine). Vertical grey lines indicate the position of the measurement window. (c,d) Normalized response as a function of concentration of selected antagonists. (e,f) Normalized response as a function of concentration of selected agonists. Data are expressed as mean and standard deviation of all individual data points from three separate experiments. Lines are Hill function fits to the data.