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. 2019 Aug 28;13:55. doi: 10.3389/fncir.2019.00055

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Copyright © 2019 Milinkeviciute, Henningfield, Muniak, Chokr, Green and Cramer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal analysis of polyinnervated neurons in the presence or absence of microglia. (A,B) Images showing reconstructed RDA-labeled calyces of Held (magenta) terminating on MNTB principal neurons in control mice at P8. Both are examples of polyinnervated MNTB neurons as evidenced by RDA-labeled calyces and additional PV (green) labeling (asterisks) outside of RDA-labeled calyces. (C,D) Examples of polyinnervated neurons in BLZ945-treated mice at P8. (E,F) Examples of polyinnervated neurons in control mice at P13. Asterisks indicate VGluT1/2 labeling on neurons in addition to RDA-filled calyces of Held. (G,H) Images of polyinnervated neurons in BLZ945-treated mice at P13. Scale bar in panel (A) = 3 μm, applies to panels (A,B). Scale bar in panels (C,D) = 3 μm. Scale bar in panel (E) = 3 μm, applies to panels (E–H).