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. 2019 Aug 28;13:55. doi: 10.3389/fncir.2019.00055

FIGURE 8.

FIGURE 8

Sequential tracing with RDA and DA488 in the VAS of wild-type and DMSO-injected mice. (A–C) Examples of polyinnervated neurons contacted by RDA- and DA488-labeled calyces of Held in wild-type mice at P8. Arrows indicate axons. (A’–C”’) Individual optical sections through the neurons shown in panels (A–C) display polyinnervation on a single MNTB neuron. (D–F) 3D reconstructions of two separate calyceal inputs contacting the same neuron in the MNTB of DMSO-treated mice at P8. (D’–F”’) Optical sections through the neurons shown in panels (D–F). (G–I) 3D images of reconstructed RDA- and DA488-filled calyces synapsing on a single MNTB neuron in DMSO-injected mice at P13. (G’–I”’) Sections through the neurons in panels (G–I) revealing multiple calyceal inputs. Scale bars in panels (A,C,D) = 3 μm. Scale bars in panels (A’,C’) = 2 μm and apply to panels (A’–A”’,C’–C”’). Scale bar in panel (B) = 4 μm. Scale bar in panel (B’) = 2 μm and applies to panels (B’–B”’). Scale bars in panels (D,F) = 3 μm. Scale bar in panel (D’) = 2 μm and applies to corresponding panels (D’–F”’). Scale bars in panels (G,H) = 3 μm. Scale bars in panels (G’,H’) = 2 μm and apply to panels (G’–H”’). Scale bar in panel (I) = 5 μm. Scale bar in panel (I’) = 3 μm and applies to panels (I’–I”’).