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. 2019 Aug 28;10:1112. doi: 10.3389/fphys.2019.01112

TABLE 1.

(A) The primer of the validation of differentially expressed circRNAs and target mRNA base sequence and (B) The primer of the miRNAs base sequence.

Regulation Primer name Sense primer (5′–3′) Antisense primer (5′–3′) Base number
(A)
Up circRNA_011190 CATCATGGCTGGATCCACGAC GAGGCTTTGGAGTGCCGTAAT 21
Up circRNA_014301 GAGTCGCTCCTGCACGAGTTG GCTGGCATTCTTGCAGTACCG 21
Up circRNA_016353 GAGGTTGTGAACAGGCTTGAT GCGTTCCTTTCTTCAAACTCC 21
Up circRNA_003259 TACACAGCACCGAGATACCAG GTACTCTTTGGAGCACCAGGC 21
Up circRNA_002985 AGAACTCGATGCTATCATGGG CTGCACTGAAGGGCCAAGTAT 21
Down circRNA_010393 TTCGAGGCCAGTGTAGCGTTG CCTTGCTCTGTCTGGGGCTAG 21
Down circRNA_009879 GGTGTCAGTGCTGGTGATGTG AAAGACACCAGTTTCCGACAG 21
Down circRNA_005414 AATTGAAGATCGGCTGCATGG AGTGCCATCGATAAACCACTG 21
Down circRNA_002960 GCTATGAGGCCTCCCTGGATAC AAAGGCTCCACGGTGAAAGG 21
Down circRNA_37695 AATCCGGAAGGAAGCTTGCTG CCAGACTGCATTCCGTGTCAT 21
Fabp3 TGGGAGTAGAGTTTGACGAGG CCCGTCCCACTTCTGCACATG 21
Cd93 GATTCCAGAGCGAGCAGAGAG AGTGGCAGCTGACTATGTTTC 21
Dusp7 AATCCCCATCTCTGACCACTG TGCTAAGCAGTGCACCAAGAC 21
Lppr4 ACCCAAGAGGCCATTCCATTC AGAGGCAGCAGTACAGAATCC 21
Stard13 CATTACCAGACACAGGGGACC GGCTTTCTATAGACAGTGCCT 21
Hif-1α CACAACGTGAGCTCCCATCTT AGGGCTTTCAGATAAAAGCAG 21
Helz2 GGTGAAGAGCCGGAGAAACTG CCCTCCCGACTTATCATGGAG 21
Midlip1 TTCAAGCAAGCAGCCAAGAAC CTGGCAAAAGTGCATCTGTTC 21
Mfap4 GATTCAACGGCTCAGTGAGTT TTCTGCTTCAGTGTCAGGAGG 21
Hnrnpa3 ATTTTGAGAAATGGGGCACAC AGTAGGTCACAAAACCAAAGC 21
Lin28b GAGCACTGAGAACCAGGGGAT CTGCTCTAAGCTTTGGCTCAC 21
Cand1 GAGCGCTTCGTACCACATCTC TCGCTGTCATCATCCAGCTTG 21
Mlxip GGAAGAGTCGCATCGAGATTG CCGTGTTTATGCCAATACAGC 21
Sec31a GCCCAGAACCACCCCATTTAC TCTCAAGGGAAGCGTTGGTAC 21
Zbtb20 GTGCTGAGAGTCTCCCAGTCG GAATCCTGGATGCCTGGGAAC 21
Hyou1 GCCTTGGTGGCTGTCCTCTTG CTTGACAATGGCCACCTTCAT 21
Lphn3 CCACCTCAGCTGTTCATCCTC TGTGCTCAGCATGTCGCAGTG 21

miRNA name Gene-specific primer (5′–3′) Reverse primer (5′–3′) Length of product (bp)
(B)
rno-miR-23a-5p ATTGGGGTTCCTGGGGAT GTGCGTGTCGTGGAGTCG 89
rno-miR-20b-3p GGGGTTCTGCAGTGTGAGC GTGCGTGTCGTGGAGTCG 63
rno-miR-3561-3p GGGGTTATCCAGGGTAGACA GTGCGTGTCGTGGAGTCG 65
rno-miR-3575 GGGGAGAACTGCTGGGTAA GTGCGTGTCGTGGAGTCG 64