TABLE 2.

OTUs obtained from bacterial isolates cultured from CCA samples and their best match among the ASVs obtained from NGS.

Culture seq and matching amplicon seq	Nb of isolates	Best match Ez Biocloud	Similarity	Closest relative species	Family	Best match NCBI	Similarity	Origin or bacterial strain	Reference
Healthy
Culture 1/ASV 2046	6	jgi.1108058	96.61	Loktanella sediminum	Rhodobacteraceae	JQ179309	99%	crustose coralline algae	Webster et al., 2013b
Culture 2/ASV 1404	1	KC311338	97.10	Loktanella sediminilitoris	Rhodobacteraceae	KT121442	99%	Loktanella sp. AODO14	Saha et al., 2016
Culture 3/ASV 277	3	AY461441	99.75	Erythrobacter aquimaris	Erythrobacteraceae	NR_025789	99%	sea water	Yoon et al., 2004
Culture 4/ASV 29690	3	AB193438	98.82	Tateyamaria omphalii	Rhodobacteraceae	JQ178889	99%	CCA under therml stress and acidification	Webster et al., 2013b
Culture 5/ASV 21203	5	JAMD01000037	97.73	Sulfitobacter pseudonitzschiae	Rhodobacteraceae	KF799150	99%	tunicate Ciona intestinalis	Dishaw et al., 2014
Culture 6	2	CCJW01000022	99.80	Vibrio crassostreae	Vibrionaceae	KF577065	100%	coral Oculina patagonica	Rubio-Portillo et al., 2014
Culture 7	3	AB920327	96.89	Spongiivirga citrea	Flavobacteriaceae	NR_134814	97%	sponge Tethya sp.	Yoon et al., 2015
Culture 8	3	AJ842344	98.37	Photobacterium rosenbergii	Vibrionaceae	KX279509	99%	coral Oculina patagonica	Rubio-Portillo et al., 2017
Culture 9	1	KF740535	98.09	Lutimonas halocynthiae	Flavobacteriaceae	AM990866	98%	sea water	unpublished
Culture 10	3	DQ781321	98.37	Sphingorhabdus litoris	Sphingomonadaceae	KY787182	99%	seagrass Posidonia oceanica	Blanchet et al., 2017
Culture 11	2	JN578481	99.75	Bacillus coreaensis	Bacillaceae	KY436466	99%	mangrove plants	unpublished
Culture 12	2	CYPU01000053	99.64	Ruegeria atlantica	Rhodobacteraceae	KY787183	100%	seagrass Posidonia oceanica	Blanchet et al., 2017
Culture 13	1	GU391222	92.49	Ferrimonas sediminum	Ferrimonadaceae	KT731259	99%	seaweed Delisea pulchra	Kumar et al., 2016
Diseased
Culture 14	3	CP000830	95.33	Dinoroseobacter shibae	Rhodobacteraceae	KF179643	98%	coral Porites	Séré et al., 2013
Culture 15/ASV 1490	3	jgi.1108064	98.61	Loktanella maricola	Rhodobacteraceae	AY576770	99%	Ruegeria sp. 3X/A02/236	Agogué et al., 2005
Culture 16/ASV 832	3	ACCU01000015	100	Labrenzia alexandrii	Rhodobacteraceae	FJ202588	99%	Orbicella faveolata	Sunagawa et al., 2009
Culture 17/ASV 3341	3	KC708867	96.96	Pelagicola litorisediminis	Rhodobacteraceae	AY612764	97%	Sea surface	Agogué et al., 2005
Culture 18/ASV 277	2	JMIW01000006	99.50	Erythrobacter longus	Sphingomonadaceae	AM691106	100%	Hypersaline spring system	Csotonyi et al., 2008
Culture 19/ASV 22655	3	jgi.1055366	97.81	Marinovum algicola	Rhodobacteraceae	FJ203203	98%%	O. faveolata diseased tissue	Sunagawa et al., 2009
Culture 20/ASV 2095	3	HG764424	92.43	Fodinicurvata halophila	Rhodospirillaceae	JQ179348	99%	crustose coralline algae	Webster et al., 2013b
Culture 21	3	ATUP01000002	98.99	Oceanicaulis alexandrii	Hyphomonadaceae	EF123309	99%	Black Band Disease coral tissues	Sekar et al., 2008
Culture 22	3	CYPU01000053	98.86	Ruegeria atlantica	Rhodobacteraceae	KY787183	99%	seagrass Posidonia oceanica	Blanchet et al., 2017
Culture 23	3	KY497472	96.44	Alteromonas aestuariivivens	Alteromonadaceae	KY515288	99%	marine env	unpublished
Shared
Culture 24/ASV 1224	5	CP002623	97.98	Roseobacter litoralis	Rhodobacteraceae	KT952700	99%	surgeon fish Acanthurus nigrofuscus	Miyake et al., 2016
Culture 25/ASV 1933	3	jgi.1108058	97.22	Loktanella sediminum	Rhodobacteraceae	FJ203462	99%	coral Orbicella faveolata	Sunagawa et al., 2009
Culture 26	2	CYPU01000053	100	Ruegeria atlantica	Rhodobacteraceae	DQ888840	99%	sponge	Muscholl-Silberhorn et al., 2008
Culture 27	3	KF740534	99.87	Ruegeria meonggei	Rhodobacteraceae	KY787161	99%	seagrass Posidonia oceanica	Blanchet et al., 2017
Culture 28	3	CP013187	100	Pseudoalteromonas phenolica	Pseudoalteromonadaceae	GQ406775	100%	diseased gorgonian	Vizcaino et al., 2010