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. 2019 Aug 28;10:906. doi: 10.3389/fphar.2019.00906

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Copyright © 2019 Su, Guo, Huang, Liu, Zhang, Sun, Li, Pang, Wang, Chen, Liu, Chen, Chen and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Thin-layer chromatography of standard β-carotene (lane 1), standard fucoxanthin (FX) (lane 2), the purified FX sample of ND-8 (lane 3), and the methanol and acetone extract of ND-8 (lane 4) on a silica plate. (B–C) High-performance liquid chromatogram and mass spectra of standard FX and ND-8 FX eluted by the silica gel column chromatography; the mobile phases used were methanol and deionized water, the mobile phase flow rate was 0.3 ml.min^–1. Retention times: standard FX, 6.41 min; ND-8 FX, 6.02 min. The diagnostic ions of standard FX are at m/z 641.46 (M + H-HOH)⁺, 681.81 (M + Na)⁺, and 1,339.83 (2M + Na)⁺. The diagnostic ions of ND-8 FX are at m/z 641.46 (M + H-HOH)⁺, 681.80 (M + Na)⁺, and 1,339.83 (2M + Na)⁺.