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. 2019 Aug 28;10:906. doi: 10.3389/fphar.2019.00906

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Copyright © 2019 Su, Guo, Huang, Liu, Zhang, Sun, Li, Pang, Wang, Chen, Liu, Chen, Chen and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of FX on NF-Κb signaling in LPS-treated RAW264.7 cells and LPS-treated mice. (A) Western blot analyses were performed to detect the levels of MyD88, IKK,p-IKK, IκBα, p-IκBα, NF-κB (p65), and p-NF-κB (p65) in LPS (1.0 mg/L)-treated RAW264.7 cells; (C) Protein levels of IκBα, p-IκBα, NF-κB (p65) and p-NF-κB (p65) in liver homogenates were evaluated by western blot analysis after LPS (20 mg/kg) challenge 6 h with or without FX treatment. (B and D) Densitometric analysis of the relevant bands was performed. The data were analyzed by the one-way ANOVA, followed by Tukey’s post hoc test. (###) p < 0.001, significantly different when compared with that of the control group. (∗) p < 0.05 and (∗∗∗) p < 0.001, significantly different when compared with that of the LPS group, ns: not significant. The data are representative of three independent experiments.