FIGURE 4.

The sulfate assimilation pathway is enhanced by malate utilization and negatively affected by CRP, but not significantly influenced by anaerobiosis. The sulfate assimilation pathway converts inorganic sulfate to sulfide and enter L-cysteine and L-methionine biosynthetic pathways, with the corresponding ORF associated with each enzymatic step. The transcriptomic data were collected from the same treatment as indicated in Figure 3. The log₂ ratio of different treatments is shown alongside the ORF.