FIGURE 5.

The respiratory chains (including electron donors, electron acceptors for aerobic respiration and electron acceptors for anaerobic respiration) of L. hongkongensis were enhanced by malate addition while reduced under anaerobic condition, which were partial mediated by CRP and FNR respectively. The relative expression of genes encoding respiratory chains were compared in WT strain under malate present (WT + M) and absent conditions (WT-M) (A–C), and between WT and Δcrp strains with malate supplementation (WT + M; Δcrp + M) (D–F). The transcriptional profiles of these genes were also compared in WT strain under aerobic (WT_AE) and anaerobic conditions (WT_AN) (G–I), and between WT and Δfnr strains under anaerobic conditions (WT_ AN; Δfnr_AN) (J–L). Each dot is representative of a single gene. The dashed lines represent a twofold over-expression (black, upper line), equal-expression (red, middle line) and –2-fold expression (black, lower line) of ratio of axis Y/X defined by log₂ of FPKM.