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. 2019 Sep 3;9:12691. doi: 10.1038/s41598-019-49147-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of pluripotency factors is decreased in nup155 deficient ESCs. (a,b) Representative images of OCT4 (red), Lamin B (green), and DAPI (blue) fluorescence signal depicting OCT4 intranuclear localization with overall decrease in signal profile in nup155^+/− (right panel) compared to ESC colonies. Bottom panel represents individual cell intensity profiles. (c,d) Maximum (Max) intensity of OCT4 in nup155^+/− ESC colonies was significantly decreased compared to WT, along with the OCT4:DAPI signal ratio (*p < 0.001). (e,f) SOX2 (red), Lamin B (green), and DAPI (blue) triple staining of nup155^+/− and WT ESC colonies show no overall change in intranuclear signal profile, as well as (g) no change in SOX2 max signal intensities between conditions (p = 0.42). (h) SOX2:DAPI signal ratio is higher in NUP155 deficient cells compared to WT. (i,j) NANOG (red) fluorescent signal profile was diminished in nup155^+/− compared to WT. (k,l) NANOG (red) max intensity and NANOG:DAPI ratio were significantly decreased in nup155^+/− ESC colonies compared to WT (*p < 0.001).