Figure 3. The effect of IVIg in OC differentiation by Th17 condition prestimulated CD4⁺ T cells. CD14⁺ monocytes were pretreated. (A) CD4⁺ T cells were pretreated with IVIg for 1 h, then cultured with Th17 condition for 72 h and then cocultured with CD14⁺ monocytes in the presence of M-CSF. (B) The gene expression of TRAP, calcitonin receptor, MMP-9, NFATc1, cathepsin K, OC-STAMP, and DC-STAMP from differentiated OCs was measured by real-time PCR. Data were normalized to beta-actin and reported in relative expression units.

DC, dendritic cell.

^*p<0.05; ^**p<0.01; ^***p<0.001.