Figure 4. Effect of IVIg on CD4⁺ T cells isolated from the PBMCs of healthy donors and cultured under Th17-polarizing conditions. Human PBMCs (n=6) were isolated from healthy subjects and pre-incubated with IVIg (0.1, 1, 10, and 20 mg/ml) for 1 h, and then cultured under Th17-polarizing conditions for 72 h. (A) CD4⁺ T cells were gated for further analysis. Next, the percentage of (B) IL-17⁺/CD4⁺ T cells, IFN-γ⁺/CD4⁺ T cells, and CD25^highfoxp3⁺/CD4⁺ T cells was measured by flow cytometry. The production of (C) IL-17 and IFN-γ by Th17-polarizing CD4⁺ T cells and secretion into the culture supernatant by ELISA.

^*p<0.05; ^**p<0.01; ^***p<0.001.